Purification and Characterization of Chloroplast Dehydroascorbate Reductase from Spinach Leaves
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چکیده
منابع مشابه
Pea chloroplast glutathione reductase: purification and characterization.
Glutathione reductase (EC 1.6.4.2) was purified from intact pea (Pisum sativum) chloroplasts by a method which includes affinity chromatography on ADP-agarose. Fractions from the affinity column which had glutathione reductase activity consisted of polypeptides of 60 and 32 kilodaltons. Separation of the proteins by electrophoresis on native gels showed that glutathione reductase activity was a...
متن کاملPurification and characterization of glutathione-dependent dehydroascorbate reductase from rat liver.
GSH-dependent enzymic reduction of dehydroascorbic acid to ascorbic acid has been studied in rat liver cytosol. After gel filtration of cytosol on Sephadex G-100 SF, dehydroascorbate reductase activity was recovered in two distinct peaks, one corresponding to glutaredoxin (an enzyme already known for its dehydroascorbate reductase activity) and another, much larger one, corresponding to a novel...
متن کاملChloroplast replication and chloroplast DNA synthesis in spinach leaves
Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
متن کاملChloroplast replication and chloroplast DNA synthesis in spinach leaves
Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
متن کاملChloroplast replication and chloroplast DNA synthesis in spinach leaves
Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2000
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcd035